Isolation, Identification and Cellulase Production by Bacillus Brevis from the Acacia Forest Soil
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چکیده
A Bacillus isolate AK5 which was provisionally identified as Bacillus brevis isolated from the Acacia forest soil at University of Chittagong was found to be capable of producing cellulolytic enzymes during growth on different cellulosic substrates. The isolate AK5 grown under different conditions showed that CMC was the best cellulosic substrates for inducing the synthesis of extracellular cellulolytic enzymes. The isolate also showed heavy growth at different pH, temperature, incubation period, substrate concentration, carbon and nitrogen sources during growth in liquid Winstead’s media having 1.2% CMC as cellulose substrate. Maximum level of CMCase is 143.22 U/ml at pH 6.5, 183.89U/ml at 40°C, 248.25U/ml in 1% substrate concentration, 259.49 U/ml at 2 hours incubation period, 154.67U/ml when asparagine as a nitrogen source and 105.21U/ml when CMC as a carbon source. Maximum 228.81 U/ml CMCase activities were recorded in liquid media when asparagine was used as a nitrogen source along with CMC as carbon source. The crude enzymes of the isolates were found to show highest enzyme activities i. e. CMCase 179.66 U/ml, FPase 162.71 U/ml, Avicelase 92.37 U/ml and β-glucosidase 81.53 U/ml.
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تاریخ انتشار 2015